eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2025-03-31 18 1 681 691 10.13005/bpj/3119 63645 article Santosh Nelogi 1 Kiran Kaur 2 Prashant Karni 1 Amit Porwal 3 Department of Prosthodontics, KLEVK Institute Of Dental Science, KLE Academy of Higher Education And Research, Belgavi, Karnataka, India. Malata Crescent, Success, Perth, Western Australia College of Dentistry, Jazan University, Saudi Arabia Background: The enhancement of implant surfaces to promote better osseointegration and reduce infection risks is critical in biomedical applications. Gold nanoparticles (GNP) have shown promise in improving the biological performance of implant materials. This study explores the effects of GNP-coated titanium surfaces on the proliferation, differentiation, and mineralization of MG63 osteoblast-like cells. Methods: Titanium discs were coated with gold nanoparticles synthesized using a green, environmentally friendly method. The coated surfaces were characterized using SEM, EDAX, and SPR techniques to confirm the presence and stability of the GNP. MG63 cells were cultured on these GNP-coated titanium discs, and their growth, proliferation, and differentiation were assessed using fluorescence microscopy, Von Kossa staining, vinculin focal adhesion proteins, and alkaline phosphatase (ALP) activity measurements. The antimicrobial efficacy of the GNP-coated surfaces was also evaluated against Staphylococcus aureus and Escherichia coli. Results: Titanium surfaces coated with  (GNP) at  5 ppm significantly enhanced the growth and differentiation of MG63 cells, with no evidence of acute cytotoxicity. The GNP-coated surfaces facilitated improved cell attachment, proliferation, and mineralization, indicating strong osteogenic potential. Additionally, the GNP-coated surfaces exhibited notable antimicrobial efficacy, MICrecorded at 0.3135 mg/cm³ for Staphylococcus aureus and 0.2915 mg/cm³ for Escherichia coli. Conclusion: Gold nanoparticle coatings at a concentration of 5 ppm significantly improve the osteogenic potential of titanium surfaces while also providing antimicrobial protection. These findings suggest that GNP-coated titanium implants could offer a dual benefit of enhanced osseointegration and infection prevention, making them a promising option for future clinical applications. Further in vivo studies are necessary to validate these results and understand the broader implications of GNP in biomedical implants. https://biomedpharmajournal.org/vol18no1/surface-functionalized-titanium-implant-with-gold-nanoparticles-on-osteogenic-and-antimicrobial-behavior-a-novel-preliminary-research-on-mg-63-cell-line/ Antimicrobial nanoparticles Gold nanoparticles Implant surface treatment Osteogenic activity Osseointegration