eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2025-03-31 18 1 899 908 10.13005/bpj/3138 65144 article I Nyoman Arsana 1 Ni Ketut Ayu Juliasih 1 Anak Agung Ayu Sauca Sunia Widyantari 1 I Gede Widhiantara 2 Putu Angga Wiradana 2 Department of Biology, Faculty of Information Technology and Science, University of Hindu Indonesia, Jl. Sangalangit, Tembau, Penatih, Denpasar, Bali Province, Indonesia Department of Biology, Faculty of Health and Science, Universitas Dhyana Pura, Jalan Raya Padangluwih, Dalung, North Kuta, Badung Regency, Bali Province, Indonesia Usadha is a traditional Balinese medicine system that has long been used in ancient medicine systems. However, so far there have been no reports on its safety. This study aims to examine the effect of polyherbal Usadha on hematology and blood biochemistry of Wistar rats. The study used a Completely Randomized Design with six treatments, namely polyherbal Usadha supplementation with doses of 0 mg/kg bw/day (E0), 100 mg/kg bw/day (E1), 200 mg/kg bw/day (E2), 300 mg/kg bw/day (E3), 400 mg/kg bw/day (E4), and 500 mg/kg bw/day (E5). The treatments were given for four weeks. The variables measured were complete hematology and blood biochemistry. Data were analyzed by One-Way ANOVA and continued with the Least Significant Difference test at a 95% confidence interval. Polyherbal Usadha appears to be safe for consumption without significant risk of side effects on blood parameters and liver function. Additional studies including histological analysis and mechanism of action will provide deeper insight into the therapeutic effects of polyherbal Usadha. In conclusion, polyherbal usada is relatively safe for liver and kidney function in Wistar rats. The results of this study support the safety of using Poliherbal Usadha as a candidate herbal medicine for long-term consumption. https://biomedpharmajournal.org/vol18no1/subacute-oral-toxicity-study-of-a-new-polyherbal-formulation-of-usadha-on-liver-and-kidney-function-of-wistar-rats/ Blood biochemistry Citrus amblycarpa (Hassk.) Ochse Haematology Piper nigrum L Polyherbal Usadha