eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2025-03-31 18 1 813 822 10.13005/bpj/3131 64626 article Marta Setiabudy 1 Anak Agung Gede Indraningrat 1 Kadek Suryawan 3 Ni Wayan Widhidewi 1 Anak Agung Ayu Lila Paramasatiari 1 Made Dharmesti Wijaya 2 Department of Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universitas Warmadewa, Denpasar, Indonesia Department of Pharmacology, Faculty of Medicine and Health Sciences, Universitas Warmadewa, Denpasar, Indonesia Department of Clinical Microbiology, Sanjiwani Gianyar Regional General Hospital, Gianyar, Indonesia Schefflera elliptica leaves contain secondary metabolites such as flavonoids, tannins, and saponins that have the potential as antibiofilm agents. Biofilms produced by Staphylococcus aureus are often the cause of chronic infections, especially on wounds and medical devices such as catheters. Antibiofilm activity of plant extracts is promising as an adjuvant or alternative therapy. The purpose of this study was to determine the antibiofilm activity of ethyl acetate extract of S. elliptica leaf against clinical isolates of S. aureus. This study is an analytical study with a laboratory experimental design to determine antibiofilm activity. The research samples were 18 clinical isolates of S. aureus obtained from patients undergoing examination at the hospital from June 2022 to December 2022. The research was carried out using the stages of sample preparation, extract preparation, biofilm assay with microtiter plate, and antimicrobial test with disc diffusion test method. The results of this study showed that the extract of S. elliptica leaf was able to reduce the average biofilm formation by clinical isolates of S. aureus significantly (p<0.001), both at doses of 10 µL (reduced by 12%) and 20 µL (reduced by 56.7%). Despite having antibiofilm activity, a disc diffusion test with a dose of 20 ul extract of S. elliptica leaf showed no or minimal antimicrobial activity against clinical isolates of S. aureus. Overall, the results of this study indicate that the extract of S. elliptica leaf has the potential as an antibiofilm agent against S. aureus. The results of this study are expected to be the basis of further research for the development of antibiofilm from natural materials. https://biomedpharmajournal.org/vol18no1/schefflera-eliptica-leaf-extract-as-antibiofilm-agent-against-the-clinical-isolates-of-staphylococcus-aureus-in-wound-blood-and-sputum/ Antibiofilm Clinical Isolate Extract Schefflera eliptica Staphylococcus aureus