eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2025-03-31 18 1 649 663 10.13005/bpj/3116 64460 article Eknath Ahire 1 Rahul Sable 1 Rajendra Mogal 1 Rakesh Shelke 2 Anjali Tajanpure 2 Department of Pharmacology, MET’s Institute of Pharmacy, Affiliated to Savitribai Phule, Pune University, Adgaon, Nashik, MH, India. Department of Pharmaceutical Analysis, MET’s Institute of Pharmacy, Affiliated to Savitribai Phule, Pune University, Adgaon, Nashik, MH, India. Background and Objective: The present examination was intended to assess the impact of β-carotene and turmeric extract individually, exclusively and with co-administration on antioxidant status in Allxoan instigated diabetic rats. Method: After the administration of β-carotene (20 mg/kg) and turmeric extract (40 mg/kg) exclusively and with co administration, the rodent were contemplated for blood glucose level, liver isolate for cell reinforcement compounds like Superoxide dismutase, Catalase, Lipid peroxidation, diminished glutathione and uric corrosive levels and pancreas for histopathology study. Result: All diabetic groups show significantly elevated blood glucose levels compared to the normal control, confirming hyperglycemia. Blood glucose levels increase further (300.56 ± 12.64 mg/dL), indicating worsening hyperglycemia. The combination shows the most substantial reduction in glucose levels (175.25 ± 3.80 mg/dL), nearing normal values, highlighting a synergistic effect. The combination significantly reduces LPO (36.01 ± 0.837), nearing normal levels, suggesting a powerful reduction in oxidative damage. The combination shows the highest improvement among treatments in SOD (58.97 ± 1.26), CAT (0.931 ± 0.025), GSH (32.66 ± 0.554), suggesting a synergistic effect in enhancing enzymatic antioxidant defense. The combination of β-carotene and turmeric extract (7.612 ± 0.121 g) nearly normalizes liver weight, suggesting a synergistic effect in protecting the liver from diabetes-induced damage. The simultaneous administration of these drugs significantly rejuvenates pancreatic beta cells. Conclusion: The outcomes got from this investigation gives a logical confirmation that β-carotene and turmeric separate have defensive impact against alloxan creates oxidative pressure prompted diabetes. The combination treatment shows synergistic effect. https://biomedpharmajournal.org/vol18no1/protective-effect-of-%ce%b2-carotene-and-turmeric-extract-against-alloxan-generated-oxidative-stress-induced-diabetes/ Alloxan Anti-diabetic activity β-carotene Free radicals oxidative stress Turmeric extract