eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2025-03-31 18 1 665 669 10.13005/bpj/3117 65086 article Lakshmana Rao Atmakuri 1 Prasanthi Thayi 1 Haritha Potluri 2 Ramesh Alluri 3 Satya Venkata Sakuntala Mamidi 4 Vijaya Kumar Ghanta 5 Department of Pharmaceutical Analysis, V. V. Institute of Pharmaceutical Sciences, Gudlavalleru, Andhra Pradesh, India. Department of Chemistry, Seshadri Rao Gudlavalleru Engineering College, Gudlavalleru, Andhra Pradesh, India. 4Department of Pharmacology, Vishnu Institute of Pharmaceutical Education and Research, Narsapur, Telangana, India. Department of Pharmacy, Government Polytechnic for Women, Kakinada, Andhra Pradesh, India. Department of Pharmacy Practice, KVSR Siddhartha College of Pharmaceutical Sciences, Vijayawada, Andhra Pradesh, India. This method offers a fast, specific and reliable way to measure both Sitagliptin and Simvastatin in a single tablet dosage form. It utilizes a plate with a pre-applied silica gel coating & a unique mobile phase composition for separation. Chloroform, methanol, toluene, and glacial acetic acid were combined in the ratio of 3:4:2:1 (v/v/v/v) and utilized as mobile phase. Exposure is achieved at 210 nm. This technique is linear between 62.5-700 nano gram per spot for Sitagliptin Phosphate & 25-300 nano gram per spot for Simvastatin. The LOD & the LOQ were established to be 10 nano gram per spot & 30 nano gram per spot; 8 nano gram per spot & 25 nano gram per spot respectively. Validation following ICH guidelines confirmed parameters to be within the limits. The method can detect & quantify Sitagliptin & Simvastatin within defined ranges. This approach has the potential for detection with confidence. https://biomedpharmajournal.org/vol18no1/novel-hptlc-technique-for-accurate-measurement-of-sitagliptin-and-simvastatin-in-combined-tablet-formulation/ Estimation Formulation HPTLC Simvastatin Sitagliptin