eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2025-03-31 18 1 701 712 10.13005/bpj/3121 63774 article Ruchi Yadav 1 Jyoti Prakash 1 Amity Institute of Biotechnology, Amity University Uttar Pradesh, Lucknow, UP, India. Acrylamide, a neurotoxic compound formed during high-temperature cooking processes, poses significant health risks, particularly regarding neurotoxicity. This study investigates the association between acrylamide exposure and neurotoxic effects, utilizing RNA sequencing (RNA-Seq) to elucidate the underlying molecular mechanisms. Gene expression profiles of neuronal cell models exposed to acrylamide was retrieved from ENA database with project id: PRJNA545942. RNAseq analysis was done to identify significant dysregulation of genes involved in critical cellular processes, including oxidative stress response, apoptosis, and neuroinflammation. Results revealed an upregulation of inflammatory pathway genes and a downregulation of neuroprotective factors, indicating a shift towards a pro-apoptotic and inflammatory state in response to acrylamide exposure. Among the upregulated genes, HMGSC1 and TUBB5 were particularly significant for their association with neurotoxicity pathways. TUBB5 plays a crucial role in neuronal migration and axonal guidance; abnormalities in this gene are severe neurodevelopmental disorders.  Findings indicate that acrylamide exposure activates pathways linked to neuronal cell death and impaired neuronal function, providing a clearer understanding of its neurotoxic potential. This research emphasizes RNA-Seq as a valuable tool for investigating acrylamide-induced neurotoxicity and contributes to risk assessment frameworks for chemical exposures. By advancing the understanding of acrylamide's impact on neuronal health, this study lays the groundwork for future studies to mitigate its neurotoxic effects and protect public health.     https://biomedpharmajournal.org/vol18no1/investigating-the-association-of-acrylamide-with-neurotoxicity-insights-from-rna-seq-and-adverse-outcome-pathways/ Acrylamide Adverse Outcome Pathways Neurotoxicity RNA-Seq Variant Analysis