eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2025-03-31 18 1 965 976 10.13005/bpj/3145 64964 article Zolzaya Bilegsaikhan 1 Dejidmaa Buyantogtokh 2 Erdenechimeg Chuluunbaatar 3 Anu Altangerel 2 Tserenkhand Gundsambuu 4 Tserentsoo Buyambaa 5 Department of Theory of Mongolian Medicine, International school of Mongolian medicine, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia Department of Pharmacology, Research Center, Institute of Traditional Medicine and Technology, Ulaanbaatar, Mongolia, Department of Chemistry and Technology, Research Center, Institute of Traditional Medicine and Technology, Ulaanbaatar, Mongolia, Laboratory of plant stress physiology, Botanic Garden and Research Institute, MAS, Ulaanbaatar, Mongolia. Department of Traditional Prescriptionology, International school of Mongolian medicine, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia O.vulgaris Moench is known for its medicinal properties, mainly to remove blood fever and inflammation. The extent of investigation aims to study the activity of O.vulgaris (OV), a medicinal plant, on hepatic acute injury-induced CCl4 in rats and establish the requirements of the standard for this medicinal plant. Wistar rats were induced to the CCl4 model with or without OV (112, 224, 560 mg/kg b.w) co-administration for 7 days. Identifications for standardization study were that the diagnostic cellular structures in O vulgaris were defined by light microscopy, and apigenin, luteolin, and aucubin were revealed using the TLC method.  The flavonoids were measured 4.3±0.62% and iridoid content was 4.86±0.93%. Some parameters for quality and safety of the medicinal plant were estimated as moisture 5.3±0.5%, ash 4.5±0.3%, insoluble ash in hydrochloric acid 1.2±0.1%, extractable in water 23±1.5%, total aerobic microbial 3*102 and total yeast and mold 2*10. OV administration (112 and 224 mg/kg b.w.) decreased hepatocellular damage, AST, ALT in CCl4-treated rats. OV clearly lowered the direct bilirubin and, MIP-1α and MCP-1 in the serum of CCl4-treated rats. OV treated group lowered the level of CTGF increased the concentration of SOD and reduced structural changes in liver tissue all over the acute liver injury. We concluded that the study results in the hepatoprotective activity of CCl4-caused acute injury by O.vulgaris in rats, the quality control criteria for O.vulgaris were defined, and the monograph’s draft Mongolian National Pharmacopeia for the medicinal plant was updated. https://biomedpharmajournal.org/vol18no1/hepatoprotective-activity-of-the-odontites-vulgaris-moench-herb-against-carbon-tetrachloride-toxicity-and-evaluating-its-standardization-parameters/ Apigenin Aucubin Carbon tetra chloride Hepatoprotective activity Luteolin