eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2025-03-31 18 1 749 759 10.13005/bpj/3125 64070 article Akanksha Singh Kachhawaha 1 Milda Elizabeth 1 Parvathy Hari 1 Athulya Rajan 2 Neelkant Verma 2 School of Medico-Legal Studies, National Forensic Sciences University, Gandhinagar campus, Gujarat India School of Forensic Sciences, National Forensic Sciences University, Gandhinagar campus, Gujarat India Iron oxide nanoparticles (IONPs) synthesised via green methods hold immense promise for various applications, particularly in biomedicine and environmental remediation. In this study, we present a novel approach for the green synthesis of IONPs using butterfly pea flowers, Clitoria ternatea (CT), extracted as a reducing and stabilising agent. The physicochemical properties of the synthesised nanoparticles were extensively characterised through UV-visible spectrophotometry, revealing an absorption peak centred at 297 nm, and Dynamic Light Scattering (DLS) analysis resulted in a polydispersity index (PDI) of 0.227 and Z-average of 68.41 nm respectively, with a zeta potential value of -23.4 mV. Characterisation was performed using Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR), and Energy Dispersive X-ray Fluorescence Spectrometry (EDXRF). Our results demonstrate the successful synthesis of IONPs, revealing their stability, morphology, elemental composition, and surface functionalization. Furthermore, the antibacterial activity of the synthesised IONPs against Escherichia coli (E. coli) was evaluated using the agar diffusion method, which showed inhibition of E. coli in a volume-dependent manner in the range 18-26 mm in comparison to 30 mm (positive control). These findings suggest the potential biomedical applications of these nanoparticles, particularly in antimicrobial therapy. https://biomedpharmajournal.org/vol18no1/green-synthesis-and-antibacterial-evaluation-of-iron-oxide-nanoparticles-using-clitoria-ternatea-flowers/ Aparajita Clitoria ternatea Green Synthesis IONPs