eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2025-03-31 18 1 1017 1029 10.13005/bpj/3150 65005 article Khurshida Saidova 1 Ibrokhim Askarov 2 Akmal Islomov 1 Jamshid Ashurov 1 Dilnoza Abdugafurova 1 Lazizbek Mahmudov 1 Department of Pharmacol A.Sadykov Institute of Bioorganic Chemistry of the Academy Sciences of Uzbekistan, Tashkent Department of Chemistry, Andijan State University, Uzbekistan, Andijan 3Department Pharmacy and Chemistry, Alfraganus University, Tashkent, Uzbekistan, Tashkent This study synthesized and evaluated a supramolecular complex of propolis with glycyrrhizic acid monoammonium salt (GMAS) in a 1:3 ratio, aiming to enhance its solubility, bioavailability, and therapeutic potential. The complex was characterized as a white to pale-yellow amorphous powder with high water solubility. Vibrational frequency analysis confirmed the presence of functional groups essential for bioactivity, while HPLC analysis identified significant levels of vitamins B2, B6, and C, suggesting antioxidant potential. In vivo evaluation using a carrageenan-induced paw edema model demonstrated dose-dependent anti-inflammatory activity, with a maximum inhibition of edema (60.7%) observed at 100 mg/kg. The complex significantly reduced swelling within 5 hours post-induction, comparable to conventional anti-inflammatory drugs. Acute toxicity studies classified the complex as non-toxic (LD50 > 2000 mg/kg), with no adverse effects observed at therapeutic doses. The potent anti-inflammatory activity is attributed to bioactive compounds, including flavonoids and phenolic acids, whose efficacy is enhanced by improved solubility through supramolecular complexation. These findings underscore the potential of the GMAS-propolis complex as a safe, natural alternative to conventional therapies for managing inflammation and oxidative stress. Future studies should focus on clinical trials and formulation optimization to establish its therapeutic applicability. https://biomedpharmajournal.org/vol18no1/development-and-hplc-characterization-of-a-water-soluble-supramolecular-complex-of-propolis-and-glycyrrhizic-acid-monoammonium-salt-composition-toxicity-and-pharmaceutical-potential/ Anti-inflammatory activity (GAMS) Glycyrrhizic acid monoammonium salt Pharmacological activity Propolis synergism