eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2025-03-31 18 1 693 700 10.13005/bpj/3120 63722 article Sandhya Parameswaran 1 Rohan Rajkumar 1 Department of Quality Assurance, Saraswathi Vidya Bhavan`s college of Pharmacy, Dombivli, Maharashtra, India Nutritional therapy and phytotherapy have surfaced as new theories in healing systems, with a strong recommendation for utilising nutraceuticals and natural plant foods as stand-alone treatments or combined with allopathic medicine. This research paper presents the formulation and evaluation of a bilayer tablet comprising beetroot and isoniazid, which is a drug of choice for tuberculosis (TB). TB increases circulating hepcidin levels, impairing dietary iron absorption and systemic utilization, often leading to anaemia. Beetroot (Beta vulgaris, Family Chenopodiaceae), known for being rich in carbohydrates, folate, minerals, and vitamin C, was chosen for its potential to address this issue. Linearity curves established by UV spectroscopy showed r² values of 0.992 for isoniazid (concentration 6-16 ppm) and r²=0. 998 for iron (0.1-2 ppm). The beetroot extract was spray-dried, and on analysis, its iron content was 0.115% w/w. A 500 mg bilayer tablet was formulated, containing 100 mg of isoniazid and 250 mg of beetroot powder, equivalent to 0.287 mg of iron, using sodium starch glycolate and croscarmellose sodium as disintegrants. The tablet was evaluated based on parameters like hardness, friability, drug content, disintegration, and dissolution. The optimal formulation, which included equal amounts of both disintegrants, demonstrated a drug content of 101-102% for both isoniazid and iron, a disintegration time of 190 seconds, and a 100% release rate at the end of 45 minutes. https://biomedpharmajournal.org/vol18no1/bilayer-tablet-formulation-of-beetroot-and-isoniazid-integration-of-herbal-nutrition-and-anti-tubercular-therapy/ Anaemia Beetroot Bilayer tablet Isoniazid Tuberculosis