eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2025-03-31 18 1 581 591 10.13005/bpj/3110 64980 article Israt Jahan Bulbul 1 Sabiha Ferdowsy Koly 1 Yesmin Begum 1 Nisrat Jahan 1 Nusratun Nahar 1 Md. Siddiqul Isalm 1 Pharmacy Department, Southeast University, Tejgaon, Dhaka, Bangladesh This study evaluated the antioxidant, antimicrobial and cytotoxic activities of different fractions from the ethanolic crude extract of Sonneratia apetala fruits (Family: Lythraceae). Antimicrobial activity was assessed using the disc diffusion method with ciprofloxacin and fluconazole as reference standards. Among the fractions, the chloroform fraction demonstrated mild to moderate antimicrobial activity (8-20 mm zone of inhibition), with the highest activity against Pseudomonas aeruginosa (20 mm) and a minimum inhibitory concentration (MIC) of 32 µg. The lethality test for brine shrimp was used to assess cytotoxicity, showing LC50 values of 22.41 µg/ml (n-hexane), 20.53 µg/ml (chloroform), and 24.60 µg/ml (ethyl acetate), compared to vincristine sulfate (8.66 µg/ml). Antioxidant activity, determined via DPPH radical scavenging, revealed IC₅₀ values of 42.03 µg/ml (n-hexane), 13.76 µg/ml (chloroform), and 49.99 µg/ml (ethyl acetate), with ascorbic acid as the standard (IC₅₀ 45.47 µg/ml). The highest amount of total phenolic content was detected in the chloroform fraction (26.88 mg GAE/g of extract). These findings suggest the chloroform fraction of S. apetala possesses significant antioxidant and cytotoxic potential, alongside moderate antimicrobial activity. https://biomedpharmajournal.org/vol18no1/assessment-of-antimicrobial-cytotoxic-and-antioxidant-potentials-of-n-hexane-chloroform-and-ethyl-acetate-extracts-of-sonneratia-apetala-banks-fruits/ Antimicrobial Antioxidant Cytotoxic Pharmacological Activity Sonneratia apetala