eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2025-03-31 18 1 1031 1042 10.13005/bpj/3151 65178 article Abdelaziz Koussoube 1 Filkpièrè Léonard Da 2 Basile Tindano 1 Tééganimbé Jean Luc Kabore 1 Elisabeth Ouedraogo 1 Balé Bayala 1 Department of Animal Biology and Physiology, Animal Physiology Laboratory, University Joseph KI-ZERBO, Ouagadougou, Burkina Faso Faculty of Science and Technology, Life and Earth Sciences Laboratory, University Norbert Zongo, Koudougou, Burkina Faso Lannea acida is a medicinal plant used in treatments for diabetes and infertility. This study's purpose was to evaluate the safety of Lannea acida trunk bark. Phytochemical screening was performed on the hydroethanolic extract of Lannea acida trunk bark. Acute innocuity was assessed in female NMRI mice with a limited dose of 2000 mg/kg bw (body weight). Subacute safety was investigated too in female Wistar rats throughout 28 days by daily administration. Four groups of six rats were formed and received distilled water and the extract at 40, 100, and 200 mg/kg bw respectively. Two other groups served as satellites and received water and a higher dose. Phytochemical screening revealed the presence of tannins, polyphenols, coumarins, alkaloids, triterpenes, and steroids. The quantitative assay revealed polyphenols, flavonoids, and tannins levels 649.77±0.36 mg EAG/g, 7.46±0.13 mg EQ/g, 79.71 ± 2.78 mg EAT/g, respectively. The extract administered as a single dose did not cause any mortality. The LD50 is greater than 2000 mg/kg. At repeated doses for 28 days, the extract did not show any significant (p>0.05) variation in animal weight. No dose of extract caused a significant (p>0.05) variation in relative organ weights. Blood counts showed no significant (p>0.05) variation. The following biochemical parameters did not vary significantly (p>0.05): Urea, creatinine, total bilirubin and direct bilirubin, triglycerides, total cholesterol, AST, and ALT. The liver functions of the rats were not affected. This study shows that the hydroethanolic extract of Lannea acida trunk bark is not toxic for the single and medium term. https://biomedpharmajournal.org/vol18no1/acute-and-subacute-toxicity-of-lannea-acida-a-rich-hydroethanolic-trunk-bark-extract-on-wistar-rats-and-nmri-mice/ Acute Toxicity Hydroethanolic Extract Lannea Acida LD50 Subacute Toxicity