eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2024-12-30 17 4 2491 2500 10.13005/bpj/3042 61804 article Putu Indah Budi Apsari 1 Putu Khrisna Dharma Jaya 2 Pande Made Alitta Cantika Putri Nadya Dewi 2 Desak Putu Oki Lestari 3 Department of Parasitology Faculty of Medicine and Health Sciences, Warmadewa University, Denpasar, Indonesia Medical Study Program, Faculty of Medicine and Health Sciences, Warmadewa University, Denpasar, Bali, Indonesia Department of Anatomical Pathology, Faculty of Medicine and Health Sciences, Warmadewa University, Bali, Indonesia Malaria is a disease that still haunts Indonesia today. The high level of anti-malarial resistance and the severity of the disease cause high mortality in malaria cases. Previous research found that Moringa oleifera can eliminate the Plasmodium berghei parasite and is an immunomodulator in mice. But how moringa works at the cellular level is still unclear. This study aimed to analyse effect of Moringa oleifera treatment in lipid vacuolization of liver, number of pyknosis cell, and organ enlargement in Mus musculus infected by Plasmodium berghei. True experimental design use 40 mice were divided into 5 group: negative control, positive control treated by dehydroartemisinin piperaquine, group 1,2, and 3 treated by 25%, 50%, and 75% Moringa extract respectively. Parasite count was determined by blood smear with giemsa staining, and their organs were collected for histopathological analysis via hematoxylin-eosin staining. Lipid vacuolization, pyknotic cell of liver was observed under light microscope. Index of Liver, spleen, and kidney were examiden by organ weight per mice body weight using digital scale. All data tested by One-Way ANOVA. The results of the study stated that Moringa oleifera reduce lipid vacuolization and reduce index of the spleen and kidney organs. However, there were no significant difference effect of Moringa oleifera in liver index and pyknotic cell. As conclusion Moringa oleifera reduce lipid vacuolization, pyknotic cell and organ enlargement in mus musculus infected by Plasmodium berghei. https://biomedpharmajournal.org/vol17no4/moringa-oleifera-reduce-lipid-vacuolization-pyknotic-cell-and-organ-enlargement-in-mus-musculus-infected-by-plasmodium-berghei/ Histopathology lipid vacuolization malaria Moringa oleifera Organ index Nuclear pyknosis