eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2024-09-30 17 3 2035 2042 10.13005/bpj/3005 60995 article Ishmuratova Aziza Saydullayevna 1 Abdugafurova Dilnoza Gulyamovna 1 Islomov Akmal Hushvaqovich 1 Mahmudov Lazizbek Umarjonovich 1 Baratov Ko’zijon Rabbim Ugli 1 Azimova Aziza Qodirali Kizi 1 A. S. Sadykov Institute of Bioorganic Chemistry of the Academy of Sciences of Republic Since ancient times, madder dye (Rubia tinctorum L.) has been used in Uzbekistan to help alleviate stone deposits in organs. In the experiment we conducted, we aimed to demonstrate that this plant can prevent the occurrence of kidney stone disease in rats using an induced model of the disease. The kidney stone disease model in rats was created by administering ethylene glycol and ammonium chloride to the animals over a period of 10 days. The substances contained in the madder dye plant mitigated the effects of the substances used to induce the disease. According to the study results, simultaneous oral administration of the madder dye extract prevented the development of kidney stone disease in rats. The extract of Rubia tinctorum L. was found to be highly effective, showing a significant nephroprotective effect due to its specific and rich composition of terpenes and terpenoids. https://biomedpharmajournal.org/vol17no3/the-study-of-the-biologically-active-effect-of-the-rubia-tinctorum-l-plant-on-rats-with-experimental-kidney-stone-disease-and-issues-of-introduction/ Ethylene Glycol Histopathology Medicinal Plants Rhizome of Plants Rubia Tinctorum L. Urolithiasis