eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2024-09-30 17 3 1647 1653 10.13005/bpj/2972 59760 article Marisca Evalina Gondokesumo 1 Faisal Akhmal Muslikh 2 Faculty of Pharmacy, University of Surabaya, Surabaya, Indonesia. Jl. Raya Kali Rungkut (Tenggilis), Surabaya, Indonesia Faculty of Pharmacy, Bhakti Wiyata Health Sciences Institute, Kediri, Indonesia. Jl. KH Wachid Hasyim No.65, Kediri, East Java, Indonesia Stunting is a chronic nutritional problem that occurs during the first 1000 days of life which is considered a golden window of opportunity. Indonesia has the highest prevalence compared to other middle-income countries. Keluwih (Artocarpus camansi) is known to have various compounds that are beneficial for the body such as anti-inflammatory and antioxidant. This study was conducted to determine the potential of the ethanol extract of Keluwih leaves (Artocarpus camansi) zebrafish stunting model against inflammatory markers, growth factors and body size. Artocarpus camansi leaves were extracted using the maceration method for 3x24 hours with 96% ethanol solvent. Zebrafish larvae were obtained from male and female broodstock (2:1), then induced using rotenone and ethanol extract of Artocarpus camansi leaves, then immunohistochemical staining was performed using growh factor (VEGF and TGF-β), inflammation (IL-6 and TNF-α) and body length measurements on day 9 dpf. The results showed that rotenone can provide a picture of stunting in zebrafish larvae from observations of growth factors, inflammation and body length, by administering ethanol extract of Artocarpus camansi leaves this can improve stunting conditions due to administration of rotenone, the concentration of ethanol extract of Artocarpus camansi leaves 2.5 ppm is the optimal concentration in improve stunting conditions. The ethanol extract of Artocarpus camansi leaves can improve stunting conditions by increasing the expression of growth factors, decreasing pro-inflammatory cytokines and improving body length in zebrafish larvae. https://biomedpharmajournal.org/vol17no3/the-effect-of-keluwih-artocarpus-camansi-leaves-extract-on-pro-inflammatory-expression-growth-factors-and-bodies-in-zebrafish-larvae-danio-rerio-stunting-model/ Artocarpus camansi Stunting Growth factors Malnutrition pro-inflammatory cytokines