eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2024-09-30 17 3 1987 1996 10.13005/bpj/3001 60162 article Abdullah H. Maad 1 Abdullah H. AL-Gamli 2 Khaled Sh. Shamarekh 3 Moath Refat 4 Mohammed E. Shayoub 5 Department of Pharmaceutical, College of Pharmacy, University of Al-Ameed, Karbala, Iraq Department of Pharmaceutics, Faculty of Pharmacy, University of Science and Technology, Yemen Department of Pharmaceutics, Faculty of Medical Science, Al-Nasser University, Sana’a, Yemen Department of Biochemistry and Molecular Biology, The Key Laboratory of Environment and Genes Related to Disease of Ministry of Education, Health Science Center, Xi’an Jiaotong University, Xi’an, China. Department of Pharmaceutics, Faculty of Pharmacy, Khartoum University, Khartoum city, Sudan Colorectal cancer ranks as the fourth most frequent cause of cancer-related fatalities on a global scale. The present study aims at assessing the anti-proliferative and apoptotic effects of Solenostemma argel extract on colorectal cancer cells (HCT-116). The antiproliferative activity was investigated using Sulfo-Rhodamine-B (SRB) assay and the apoptotic effects were demonstrated utilizing acridine orange/ ethidium bromide (AO/EB) staining method. The antiproliferative results demonstrated that the extract exhibited dose-dependent antiproliferative activity, with an IC₅₀ value of 85.3 μg/ml. The apoptosis results clearly demonstrated the ability of the methanolic extract of Solenostemma argel in inducing apoptosis in HCT-116 cancer cells. In conclusion, the investigation highlights the considerable antiproliferative and apoptotic impacts of Solenostemma argel leaf extract on HCT-116 colorectal cancer cells. This underscores its potential as a promising chemopreventive agent specifically targeting HCT-116 colon cancer cells. https://biomedpharmajournal.org/vol17no3/antiproliferative-and-apoptotic-effects-of-solenostemma-argel-leaf-extracts-on-colon-cancer-cell-line-hct-116/ Antiproliferation Apoptosis Colorectal cancer HCT-116 Solenostemma argel