eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2024-09-30 17 3 1795 1806 10.13005/bpj/2984 61350 article Yogita Ale 1 Shilpa Rana 2 Vikash Jakhmola 3 Kapil Kumar 4 Ritik Singh Rana 1 Diksha Rawat 1 Department of Pharmaceutics, Uttaranchal Institute of Pharmaceutical Sciences, Uttaranchal University, Dehradun, India Department of Pharmacology, Uttaranchal Institute of Pharmaceutical Sciences, Uttaranchal University, Dehradun, India Department of Pharmaceutical Chemistry, Uttaranchal Institute of Pharmaceutical Sciences, Uttaranchal University, Dehradun, India Samrat Prithviraj Chauhan College of Pharmacy, Kashipur, Uttarakhand Technical University, Uttarakhand, India Breast cancer (BC) is one of the most severe cancers among women globally. Local recurrence of cancer after surgery is usually seen as a poor predictor of prognosis. Cancer treatment has been significantly transformed by the progress made in nanotechnology, and nanoparticles have emerged as pivotal components in this domain. Metal nanoparticles are produced using plant extract in green synthesis or eco-friendly techniques for the stabilizing and reducing substance. The current research study synthesizes silver nanoparticles (AgNPs) from Epipremnum aureum leaf extract using the green synthesis method and its evaluations using UV-Vis, FTIR, XRD, SEM, and EDX characterization techniques. The EA-AgNPs exhibit a significant absorption peak at wavelength 420 nm, which confirms the AgNP's presence by UV-visible spectrometer. FTIR spectrum reveals the strong band at 1586.020 cm^-1 confirming the O-H group presence. The stretching and bending modes of vibration of the NO₃^2- a sharp band represented molecule at 1382.987 cm^-1 and a very tiny band at 1272.241, 1077.355 cm^-1. The XRD spectrum exclusively showed Ag peaks, with no additional chemical contaminants, signifying the sample's purity, and the average particle size was 12.92 nm.  MTT assay result observed high cytotoxic activity of  EA-AgNPs against MCF-7 cells with IC₅₀= 0.1106 µg/ml.  This research study aims to preliminary investigate the in-vitro antiproliferative activity of green process synthesized Epipremnum Aureum silver nanoparticles (EA-AgNPs) against breast cancer cell line (MCF-7). https://biomedpharmajournal.org/vol17no3/antiproliferative-activity-of-green-process-synthesized-epipremnum-aureum-silver-nanoparticles-against-breast-cancer-mcf-7-cells/ Antiproliferative activity Breast cancer Green synthesis MTT Assay Silver nanoparticles