eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2024-06-25 17 2 1279 1290 10.13005/bpj/2941 59039 article Nyamdemberel Tsagaanbaatar 1 Uranzaya Dashzeveg 1 Nomindari Nyamdemberel 2 Myadagbadam Urtnasan 1 Chimedragchaa Chimedtseren 1 1Institute of Traditional Medicine and Technology, Ulaanbaatar, Mongolia 2Mongolia National University of Medical Sciences, Ulaanbaatar, Mongolia This study examines ARUR-10, the herbal prescription commonly utilized in Traditional Mongolian Medicine practices for the treatment of kidney disease and the improvement of physical strength. This investigation aims to establish a profile of the biologically active compounds, antioxidant activity, and the anti-inflammatory impact on the kidneys in the traditional ARUR-10 prescription. For this analysis, we used Thin-Layer Chromatography (TLC) to identify phenolic compounds in the traditional prescription. Quantification of these bioactive substances was determined by UV/Vis spectrophotometry. The traditional ARUR-10 prescription, or traditional drug, had a higher polyphenolic content of 12.9±0.361% (gallic acid equivalent) and a sum flavonoid content of 0.33±0.015% (Rutin equivalent). The antioxidant activity was measured using the inhibiting free radical’s DPPH method and evaluated using the IC50 value. The 100 μg/ml concentration inhibited free radicals by 68.47%, whereas 200 μg/ml inhibited them by 83.29%, with an IC50 of 92.4 μg/ml. This study concluded that the traditional ARUR-10 prescription contains many polyphenolic compounds and flavonoids and has antioxidant properties. ARUR-10 has a moderate level of toxicity and no signs of chronic toxicity have been detected. Urine excretion was 1.52 ± 1.23 times higher than in the distilled water group (0.97 ± 0.71). ARUR-10 at doses of 240 mg/kg (1.62±1.14) had a similar effectiveness to Hydrochlorothiazide (1.52±1.23). In the gentamicin-induced nephritis model, ARUR-10 reduced serum creatinine by 42.8%, uric acid by 14.9%, and urea by 32% at doses of 240 mg/kg, compared to the control group that produced the pathological model. It had a protective effect on the kidneys by preserving the tube structure; cell death and cell necrosis were not observed. https://biomedpharmajournal.org/vol17no2/results-of-chemical-and-pharmacological-studies-of-the-mongolian-traditional-prescription-arur-10/ 2.2-diphenyl-1-picrylhydrazyl flavonoid nephroprotective activity phenolic compounds traditional ARUR-10 prescription