eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2024-06-25 17 2 1135 1148 10.13005/bpj/2929 58641 article Ni Putu Widya Astuti 1 Ida Bagus Putra Manuaba 3 I Made Jawi 4 Anak Agung Bawa Putra 3 Putu Angga Wiradana 5 I Gede Widhiantara 5 Doctoral Study Program, Faculty of Medicine, Universitas Udayana, Denpasar City, Bali Province, Indonesia Study Program of Public Health, Faculty of Health, Science, and Technology, Universitas Dhyana Pura, Jalan Raya Padangluwih, Dalung, North Kuta, Badung Regency, Bali Province, Indonesia Department of Chemistry, Faculty of Mathematical and Natural Sciences, Universitas Udayana, Badung Regency, Bali Province, Indonesia Department of Pharmacology, Faculty of Medicine, Universitas Udayana, Jalan P.B. Sudirman, Dangin Puri Klod, Denpasar City, Bali Province, Indonesia Research Group of Biological Health, Study Program of Biology, Faculty of Health, Science, and Technology, Universitas Dhyana Pura, Jalan Raya Padangluwih, Dalung, North Kuta, Badung Regency, Bali Province, Indonesia Traditional herbal remedies have an important role in human health. Empirically, Blumea balsamifera is often used as a traditional beverage to alleviate fever symptoms, lower cholesterol levels, and maintain body immunity. The purpose of this study was to discover the phytoconstituent profile that contributes to the anti-diabetic properties of B. balsamifera leaf extract (BBLE) using in silico approaches.LCMS/MS was used to identify the constituent profile of BBLE, and the ability of these compounds against diabetes-related proteins was analyzed computationally.Three proteins related to diabetes are NF-KB p65, GLP-1, and DPP-4. A total of 18 compounds were successfully identified through LCMS/MS, including 4 compounds known to be flavonoid derivatives and can be used as markers of BBLE. Pheophorbide A and 1,1-Cyclopentanediacetic acid were reported for the first time to inhibit the NF-KB p65, GLP-1, and DPP-4 proteins in docking simulation studies. Based on these findings, it can be confirmed that the bioactive compounds in BBLE show strong inhibitory potential against anti-diabetic proteins. https://biomedpharmajournal.org/vol17no2/phytoconstituents-analysis-and-anti-diabetic-potential-of-sembung-leaf-extract-blumea-balsamifera-l-dc-through-inhibition-of-nf-kb-p65-glp-1-and-dpp-4-proteins-with-in-silico-approaches/ Antidiabetic activity Blumea balsamifera In Silico LCMS/MS Phytoconstituent