eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2024-06-25 17 2 769 778 10.13005/bpj/2903 58065 article G Hari Krishnan 1 Sheeba Santhosh 2 G Mohandass 3 T Sudhakar 4 Department of Electrical and Electronics Engineering, Sree Vidyanikethan Engineering College, Mohan Babu University, Tirupati, Andhra Pradesh, India. Department of Electronics and Communication Engineering, Panimalar Engineering College, Chennai, India Department of Biomedical Engineering, Saveetha School of Engineering, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, India Department of Biomedical Engineering, Sathyabama Institute of Science and Technology, Chennai, India. Electrical characterization of biological tissues offers a promising non-invasive approach to understanding tissue properties. This research rigorously examines how bio-impedance values are influenced by the frequency of the input signal and the strategic placement of electrodes. Utilizing the capabilities of the AD5933 evaluation board by Analog Devices, the study spanned a frequency range from 1KHz to 200 KHz. The results highlighted a significant decrease in impedance with increasing frequency, a trend attributed to the capacitive characteristics of cell membranes. Moreover, the specific distance between electrodes was identified as a crucial factor affecting measurement accuracy. Given these findings, the adaptation of this technique in healthcare settings, particularly in diagnostics, could offer enhanced precision, underscoring its potential relevance in clinical practice. https://biomedpharmajournal.org/vol17no2/non-invasive-bio-impedance-diagnostics-delving-into-signal-frequency-and-electrode-placement-effects/ Bio-Impedance Electrode Positioning Electrical Properties Input Signal Frequency Pathological Conditions Tetra Polar Electrode