eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2024-06-25 17 2 929 937 10.13005/bpj/2913 57693 article R. Karthikeyan 1 G. Haema 1 V. Vijayan 1 V. Alamelu 1 M. Sakthiganapathy 1 S. Shanmuganathan 1 School of Pharmacy, Sri Balaji Vidyapeeth, SBV Campus, Pillayarkuppam, Puducherry, India Faculty of Pharmacy, Bharath Institute of Higher Education and Research, India Saveetha College of Pharmacy, Saveetha Institute of Medical and Technical Sciences, Chennai, India Faculty of Pharmaceutical Sciences , UCSI University,UCSI Heights, 56000 Kuala Lumpur, Malaysia Silver nanoparticles were combined with Ocimum Tenuiflorum L. green and purple (Krishna Tulasi) assortments using a single advance that was eco-friendly. Ultraviolet-Visible spectrophotometer, Fourier transform Infra-red, X-Ray Diffraction, Scanning Electron Microscope (SEM), and Energy Dispersive X-ray spectroscope was used to describe the particle nature of integrated AgNps (EDX). A variety of modifications confirmed the nanoparticle arrangement and UV-Visible spectroscopy was used to quantify the Surface Plasmon retention band. In the FTIR, the presence of the aromatic ring's C-H vibration is comparable to the presence of flavonoids and carbonyl gathering in the C-O stretch vibration. The inserted silver nanoparticles can clearly be seen under an SEM microscope, and the particles are estimated to be between l and 100 nm in size. The Energy Dispersive X-ray spectra demonstrate the nanomaterial’s quality. Union is traditional and viewed as proficient as far as response time as well as practicality. The combined nanoparticles were exposed to Anti-microbial and Anti proliferative investigations by in-vitro strategies and showed a portion of the subordinate movement against specific microbes and cancer cells. https://biomedpharmajournal.org/vol17no2/in-vitro-anti-bacterial-and-anti-cancer-activity-of-ocimum-tenuiflorum-l-leaf-extract-induced-silver-nanoparticles-a-study-of-characterization-cum-evaluation/ Anti-bacterial Anti-cancer study Characterization Ocimum tenuiflorum Particle size Silver nanoparticles Zeta potential