eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2024-06-25 17 2 1329 1334 10.13005/bpj/2946 58342 article Neevedha K 1 Anitha E 1 Thulasi Gokul 2 IsswariyaAnandan 1 S. Priestlyvivekkumar 1 Department of Pharmacology ,Panimalar Medical College Hospital And Research Institute, Poonamallee, Chennai, India Department of Pharmacology, Government Medical College, Ongole, India This study aims at evaluating the anticancer effect on the MCF-7 (Michigan Cancer Foundation-7) cell line of human breast cancer using Thymoquinone and Tamoxifen alone as well as in combination therapy by 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) Test. NCCS in Pune provided the MCF-7 cell line. The cells were kept at 37°C in a humidified medium of 50µg/ml CO₂ in Minimal Essential Medium added with 10percent FBS (Foetal Bovine Serum), streptomycin (100µg/ml), as well as penicillin (100U/ml). MTT-(3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl tetrazolium bromide) test was conducted on MCF-7 cell line for Thymoquinone and Tamoxifen as sole and combination therapy. Measurements were performed using UV (Ultra-Violet)-spectrophotometer at 570-nanometre absorbance and the content needed for a 50 percent inhibitory concentration (IC50) was calculated and evaluated graphically. IC 50 of Thymoquinone on MCF 7 was found to be at 31.2 µg/ml and Tamoxifen was at 62.5 µg/ml were as in combination therapy the IC 50 was found to be at 7.8 µg/ml. There is a remarkable reduction in concentration to achieve IC 50 percentage in combination therapy with a comparison with individual therapy. Therefore, the combination therapy of Thymoquinone and Tamoxifen on the MCF-7 cell line is more efficacious when compared to individual treatment on cell viability inhibition. https://biomedpharmajournal.org/vol17no2/comparison-of-anticancer-activity-between-thymoquinone-and-tamoxifen-thymoquinone-tamoxifen-on-mcf-7-cell-line-of-human-breast-cancer-an-invitro-study/ Inhibitory Concentration Michigan Cancer Foundation-7 3-(4 5-dimethylthiazol-2-yl)-2 5-diphenyl tetrazolium bromide Tamoxifen Thymoquinone