eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2024-03-20 17 1 271 279 10.13005/bpj/2855 55636 article Devyani Diah Wulansari 1 Dwi Winarni 2 Lanny Sapei 3 Marisca Evalina Gondokesumo 4 Raden Joko Kuncoroningrat Susilo 5 Rochmad Indrawanto 6 Department of Clinical and Community Pharmacy, Faculty of Pharmacy, University of Surabaya, Raya Kalirungkut, Surabaya 60293, East Java, Indonesia Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya 60115, Indonesia Department of Chemical Engineering, Faculty of Engineering, University of Surabaya, Raya Kalirungkut, Surabaya, East Java, Indonesia Department of Biology Pharmacy, Faculty of Pharmacy, University of Surabaya, Raya Kalirungkut, Surabaya, East Java, Indonesia Unit of Nanotechnology Engineering, Department of Engineering, Faculty of Advance Technology and Multidiscipline, Universitas Airlangga, Surabaya, Indonesia PT Lautan Natural Krimerindo, Mojokerto, Indonesia Immunomodulators are substances that have the ability to influence the human immune system. FiberCreme is a commercial non-dairy food high in oligosaccharides, which are difficult to digest. Combination of FiberCreme and VCO (FC-VCO) considered to possess high value of bioavailability in body. This study was aimed to determine immunomodulation effect of FiberCreme-VCO against immunosuppressed rats. This study used male rat (Rattus novergicus) which was induced by doxorubicin twice a week for seven days. Treatment by FC-VCO was then administered orally for 14 days. Whole blood and spleen were collected and analyzed based on immunomodulator parameters such as spleen weight, body weight, IL-6, TNF-α, and INF-γ levels, the CD4⁺/CD8⁺ ratio and the percentage of FOXP3. Statistical analysis was determined by GraphPad Prism software (version 9: San Diego, CA, USA). Regardless of dosage, FC-VCO did not improve body weight or lymphatic weight appreciably. In contrast to the negative group, FC-VCO supplementation at a dose of 6 mg/kgBW was able to raise CD4⁺ levels, and this difference was statistically significant (p<0.05). IFN-γ levels were also increased by FC-VCO at a dose of 9 mg/kgBW, and these differences were statistically significant (p <0.05) when compared to the negative group. Since FC-VCO affects the roles and responsibilities of CD4⁺ and IFN-γ in immunosuppressive situations, it can strengthen the immune system. https://biomedpharmajournal.org/vol17no1/modulation-of-immune-response-from-fibercreme-vco-based-supplementation-in-immunosuppressed-rats/ Doxorubicin FiberCreme Healthy Life Immunomodulator Virgin Coconut Oil