eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2024-03-20 17 1 125 134 10.13005/bpj/2840 56503 article Ahmed L. Osman 1 Abd Elgadir A. Altoum 1 Devapriya Finney Shadroch 1 Asaad MA. Babker 1 Hesham Elashmouny 2 Nourhan Khaled Hassan 2 Department of Medical Laboratory Sciences, College of Health Sciences, Gulf Medical University. Ajman, United Arab Emirates Department of Pharmaceutical Sciences, College of Pharmacy, Gulf Medical University, Ajman, United Arab Emirates Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, October 6 University, Cairo, Egypt Department of Pharmaceutics and Pharmaceutical Technology, College of Pharmacy, University of Sharjah, 27272, Sharjah, United Arab Emirates Research Institute of Medical and Health Sciences, University of Sharjah, 27272, Sharjah, United Arab Emirates Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Cairo University, Cairo, Egypt Fluconazole is potent triazole drug used as effectively anti-fungal for treating a variety of local and systemic fungal infections. This drug is sparingly soluble in water. The objective of this research was to statistically optimize nanovesicular formulations contain fluconazole to improve its antifungal effect. The nanovesicular formulations were composed of Tween^® 80, cetyl alcohol, and chitosan. The prepared nanovesicular formulations were investigated for their zeta potential, polydispersity index, particle size, and encapsulation efficiency. The nanovesicular that has been optimized formulation was consist of tween/cetyl alcohol ratio of 15:1 and 0.1% chitosan. Additionally, the optimized formulation increased significantly in fluconazole release ratio and extent in comparison to the suspension form of the drug. The find that fluconazole nanovesicles had a better effect and lower MIC when compared to the aqueous fluconazole suspension. Finally, the optimized nanovesicles can be considered a promising nanocarrier for delivery of fluconazole to increase its systemic antifungal efficacy. https://biomedpharmajournal.org/vol17no1/evaluation-of-the-antifungal-activity-of-fluconazole-nanovesicles-against-aspergillus-fumigatus/ Antifungal Activity Fluconazole Central Composite Design In Vitro Release Nanovesicles