eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2024-03-20 17 1 253 262 10.13005/bpj/2853 56476 article Endang L. Widiastuti 1 Eka Ayu Lailatul Istikomah 1 Melisa Intan Barliana 2 Nuning Nurcahyani 1 Endah Setyaningrum 1 Department of Biology, Lampung University, Lampung, Indonesia Department of Pharmacy, Padjadjaran University, Bandung, Indonesia Many treatments of cervical cancer leave some side effects.  Therefore, other cancer treatment which can reduce the side effects must be elaborated, one is by searching of some natural products. Some of the natural products could be obtained from the marine biota such as seagrass, Cymodecea rotundata and Enhalus acoroides. Most of seagrass have bioactive compounds such as alkaloids, flavonoids, saponins and steroids which have antioxidants, antitumor and anticancer activity. The purpose of this study was to determine the activity of the ethanolic extraction of C. rotundata and E. acoroides against HeLa cells. To determine their cytotoxic and antiproliferative activity, we used the CCK-8 assay, with series concentrations of 62.5 ppm, 125 ppm, 250 ppm, 500 ppm, 1000 ppm and 2000 ppm, and doxorubicin as control drug (with incubation times of 24, 48, 72 hours). The results indicated that both extracts had cytotoxic effect on HeLa cell line, with IC₅₀ values of 856.65 ppm (C. rotundata) and 645.96 ppm (E. acoroides). While their anti-proliferative activity indicated by the doubling time of HeLa cell lines, reached 297 hours for C. rotundata at 1000 ppm, and 370 hours for E. acoroids at of 500 ppm. It was concluded that the ethanol extracts of C. rotundata and E. acoroides were cytotoxic and had antiproliferative activity against HeLa cells. https://biomedpharmajournal.org/vol17no1/cytotoxic-and-antiproliferative-testing-of-hela-cervical-cancer-cells-using-seagrass-ethanolic-extraction-cymodocea-rotundata-and-enhalus-acoroides/ Antiproliferative activity Cytotoxic Cymodecea rotundata Enhalus acoroides  HeLa cells