eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2024-03-20 17 1 63 69 10.13005/bpj/2834 56883 article Mariia Shanaida 1 Olesia Palamar 1 Olena Holembiovska 2 Department of Pharmacognosy and Medical Botany, I. Horbachevsky Ternopil National Medical University, Ternopil, Ukraine Department of Translational Medical Bioengineering, National Technical University of Ukraine “Igor Sikorsky Kyiv Polytechnic Institute”, Ukraine During its triple extraction, the HPLC analysis revealed polyphenols' contents in the A. foeniculum herb. Several hydroxycinnamic acids (rosmarinic, chlorogenic, ferulic and caffeic) and flavonoids (apigenin, apigenin-7-O-glucoside, hyperoside, quercitrin, rutin and quercetin) were identified in the A. foeniculum herb. It was established that rosmarinic acid followed by apigenin-7-O-glucoside and apigenin were the predominant compounds of the A. foeniculum raw material. The content of rosmarinic acid as the major compound during the primary, secondary and tertiary extraction decreased in the following order: 37.563>15.435>0.642 (mg/g); the content of apigenin-7-O-glucoside was 24.508>9.107>0.945 (mg/g) and apigenin was 19.547>9.676>1.816 (mg/g), respectively. Generally, the third extraction was determined to be inefficient in terms terms of low content of polyphenols as well as excessive analysis time and solvent costs. https://biomedpharmajournal.org/vol17no1/chromatographic-profile-of-polyphenols-in-the-agastache-foeniculum-pursh-kuntze-herb-evaluation-of-optimal-extraction-efficiency/ Aerial part Anise hyssop High-performance liquid chromatography Multiplicity of extraction Polyphenols