eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2023-12-31 16 4 2007 2019 10.13005/bpj/2777 53922 article Aravinda Pai 1 Pooja Sharma P. C 1 Venkatesh Kamath 2 Chandrashekhar K. S 3 Vasudev Pai 3 Muddukrishna B.S 4 Department of Pharmaceutical Chemistry, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education, Manipal, India Department of Pharmaceutical Biotechnology, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education, Manipal, India Department of Pharmacognosy, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education, Manipal, India Department of Pharmaceutical Quality Assurance, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education, Manipal, India In the present study, a congeneric series of novel substituted coumarin pyrazole carbaldehydes were synthesized. The compounds were characterized by various physical and spectroscopic methods. Preliminary cytotoxicity of the analogues was carried out using the MTT assay method on A-549 Lung cancer cell lines. The synthesized compounds possessed appreciable cytotoxicity against lung cancer cell lines. Out of the 8 synthesized compounds, the compound P-03 showed marked cytotoxicity of 13.5 mmol compared to standard doxorubicin which showed cytotoxicity value of 3.5 mmol. The compound P-03 was further investigated for its ability to induce apoptosis and its effect on cell cycle analysis. The compound P-03 was found to be an early apoptotic agent. After performing a cell cycle investigation, it was discovered that the compound P-03 effectively inhibited the G2/M phase of the cell cycle. https://biomedpharmajournal.org/vol16no4/synthesis-characterization-and-mechanistic-anticancer-evaluation-of-novel-analogues-of-pyrazoles-derived-from-substituted-3-acetyl-coumarins/ Anticancer Apoptosis Coumarin Cell Cycle Hybrid Mechanistic Pyrazole