eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2023-12-31 16 4 2531 2537 10.13005/bpj/2827 54563 article Ali Abd Sulaiman 1 ‏Hawraa kadhim Abbas 2 Ali Mahmoud Al-Samydai 3 Hussein K. Alkufi 4 Haneen abdul hadi Kharaba 5 Hany A. Al-hussaniy 6 Radiology Department, Al-Karama Hospital, Baghdad, Iraq Department of Pharmacy, Alzahraa teaching hospital at Al-Najaf city. Iraq College of Pharmacy, Al-Ahliyya Amman University, Amman, Jordan Department of Pharmacognosy, College of Pharmacy, Thi-Qar University, Iraq Department of pharmaceutics, college of pharmacy, University of Baghdad, Baghdad, Iraq Dr Hany Akeel Institute, Iraqi Medical Research Center, Baghdad, Iraq Background: Multiple sclerosis (MS) is a neuropathological disease diagnosed by a magnetic resonance imaging device. Early patients affected with MS are named active. Objectives: This study assessed the difference between active and non-active MS using the region of interest value. Methods: Twenty patients with MS included in this study were examined with T1 weighted image  (T1W) with and without contrast agent injection to increase the brightness of darker regions after only 10 minutes. Also, T2 weighted images  (T2W) and Fluid-attenuated inversion recovery (FLAIR) were scanned. The area of interest option was calculated for all cases. Results: The result shows that the region of interest (ROI) value was significantly higher for T1 weighted image (T1W) with contrast than without. Furthermore, the range of FLAIR values was higher than the (T2W). Conclusion: The ROI is an effective parameter for diagnosing active MS early by values instead of the radiographic picture. https://biomedpharmajournal.org/vol16no4/a-comparative-diagnostic-study-for-using-the-contrast-agent-in-active-and-non-active-multiple-sclerosis-by-region-of-interest-parameter/ Fluid-attenuated inversion recovery Lymphatic System Multiple Sclerosis Magnetic Resonance Imaging Nervous System Diseases