eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2023-06-30 16 2 809 816 10.13005/bpj/2663 48976 article Ratika Rahmasari 1 Galang Reynaldi 2 Roshamur Forestrania 2 Nuraini Puspitasari 2 Berna Elya 2 Laboratory of Microbiology and Biotechnology, Faculty of Pharmacy, Universitas Indonesia, Depok, Indonesia Laboratory of Phytochemistry and Pharmacognosy, Faculty of Pharmacy, Universitas Indonesia, Depok, Indonesia Oral and dental problems caused by microbial infections are one of the global health problems which reduce the quality of life for those affected. Enterococcus faecalis is a gram-positive bacterium, linked to oral cavity infections, which are usually managed by chlorhexidine as an oral antiseptic. However, there is a concern related to the occurrence of its resistance. Zanthoxylum acanthopodium has been reported to have antimicrobial activity, but its activity against E. faecalis has not yet been known. This research aims to observe microscopic characteristics by scanning electron microscopic, determining total flavonoid content, and identifying anti-E. faecalis effectiveness of fruit and leaf of Z. acanthopodium ethanol extract. Microscopic evaluation confirmed the presence of the epicarp and mesocarp in fruits, meanwhile three different forms of calcium oxalate existed in its leaf. Further evaluation showed that fruit ethanol extract (flavonoid content of 20.84 mg EQ/g) did not exhibit activity against E. faecalis. However, leaf ethanol extract (flavonoid content of 131.73 mg EQ/g) showed activity against E. faecalis with a coefficient value of 0.4 relative to chlorhexidine.  This study demonstrated for the first time, antimicrobial effectiveness of Z. Acanthopodium leaves ethanol extract against E. faecalis. https://biomedpharmajournal.org/vol16no2/zanthoxylum-acanthopodium-microscopic-chemical-characteristic-and-anti-enterococcus-faecalis-effectivity-of-fruit-and-leaves-ethanol-extract/ Anti- Enterococcus faecalis Flavonoid Microscopic observation Zanthoxylum acanthopodium