eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2023-06-30 16 2 927 934 10.13005/bpj/2675 48726 article Regina Tedjasulaksana 1 Ni Ketut Ratmini 2 Midwifery Department, Polytechnic of Health Denpasar, Bali, Indonesia Dental Hygienist Department, Polytechnic of Health Denpasar, Bali, Indonesia Kirinyuh leaves (Chromolaena odorata L.) are natural medicinal plants for wound healing. WHO (World Health Organization) recommends and concern about the safety of plant medicines. Due to limited  toxicity study of Chromolaena odorata,  this study aimed to determine the acute toxicity of the ethanolic extract of C. odorata  in terms of LD₅₀ and its effect on liver and kidney function of male white rats. The observed toxicity parameters were LD₅₀ and delayed toxic effects for 14 days, including toxic symptoms, body weight, AST, ALT and creatinine test, liver and kidney histopathology. The study used 24 white male rats divided into 4 groups, namely the control group NaCMC 0.5%, the treatment group of C. odorata  leaf ethanol extract 5 g/kg bw, 10 g/kg bw and 15g/kg bw with a single dose. The results showed that no white rats died during the 14 days of observation. Toxic symptoms that appear only white rats lack of appetite. Other parameter was body weight between days 1, 3, 7, 10 and 14 for each treatment group was significantly different and tended to decrease. The AST, ALT and creatinine values ​​were not significantly different for all treatments. Histopathology of the liver and kidneys showed no abnormalities. Based on these results, the LD₅₀ of kirinyuh leaf extract was 15 g/kg bw and delayed toxic effects in the form of lack of appetite which caused weight loss in all treatment groups. https://biomedpharmajournal.org/vol16no2/toxicity-effects-of-kirinyuh-leaf-ethanol-extract-chromolaena-odorata-l-on-white-rats-rattus-novergicus/ Chromolaena odorata Delayed Toxic Effect LD50 white rats