eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2023-06-30 16 2 1151 1157 10.13005/bpj/2695 48767 article Ni Nyoman Astika Dewi 1 Heri Setiyo Bekti 1 I Gusti Agung Dewi Sarihati 1 Gusti Ayu Marhaeni 2 Luh Putu Rinawati 1 Medical Laboratory Technologist, Polytechnic of Health Denpasar, Bali, Indonesia. Center of Excelence in Science and Technology, Polytechnic of Health Denpasar, Bali, Indonesia Midwifery, Polytechnic of Health Denpasar, Bali, Indonesia. Center of Excelence in Science and Technology, Polytechnic of Health Denpasar, Bali, Indonesia, Grapes are rich in bioactive molecules that can act as antioxidants, antimicrobials, anti-inflammatory and anti-cancer. Paraoxonase (PON) is an enzyme that can combine with HDL and function as an antioxidant that can protect LDL and HDL from lipid peroxidation which can prevent atherosclerosis. We used a high-cholesterol diet given to Wistar Rats to find out the effect of green grape extract (GGE) on the production of PON3 serum levels in rats. GGE was made using the maceration method. Serum PON3 levels were measured using the ELISA method and measured at 450 nm. The results showed that the highest PON3 serum levels were found in the rat group which was given a GGE dose of 500 mg/200 g BW/day (AH2), followed by levels in rats given GGE at a concentration of 250 mg/200 g BW/day (AH1). Tamhane’s test showed that there was a difference between rats that were only given a high-cholesterol diet (DTK) and AH1, AH2, and rats that were fed a standard diet (DS) with AH1 and AH2. However, no difference was found between AH1 and AH2. The higher the dose of GGE given, the higher the serum PON3 level. Further research can better analyze the active compounds in GGE in increasing serum PON3 levels. https://biomedpharmajournal.org/vol16no2/the-potential-of-green-grape-vitis-vinifera-l-extract-on-paraoxonase-3-serum-levels-in-rats-were-given-high-cholesterol-diet/ Atherosclerosis Coronary heart disease Green grape extract Paraoxonase-3