eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2023-06-30 16 2 1011 1017 10.13005/bpj/2682 48539 article Navin Patil 1 Akshatha P Kamath 2 Amrita Parida 3 Karthik Rao 4 Nadeem Khan G 5 Shanti Gurung 6 Dean of Basic Sciences, All Saints University School of Medicine, Dominica Sharavathi dental college and Hospital Shimoga Pharmacology, Kasturba medical college, Manipal, Manipal Academy of Higher Education, Manipal, India. Medicine, KS Hegde Medical Academy, Mangalore Karnataka, India Department of Cell and Molecular Biology, Manipal School of Life Sciences, Manipal Academy of Higher Education, Manipal, India 6Pharmacology, All Saints University School of Medicine, Dominica In the present study, reproductive toxicity caused by cyclophosphamide on sperm parameters, biochemical parameters, histopathology and protective effect of wheatgrass juice was examined. There were total 6 groups of adult Wistar albino rats. Each group had 6 rats. After 24hrs of the last treatment, rats were sacrificed by cervical dislocation and sperm parameters like sperm count and motility, plasma malonaldehyde (MDA) levels, catalase (CAT) levels, superoxide dismutase (SOD) and glutathione (GSH) activities in testicular tissue, and testicular histopathological changes in the testicular tissue were examined. Administration of cyclophosphamide showed an increase in plasma MDA level and decrease in SOD, GSH and Catalase activity. These values were statistically significant. However, wheatgrass juice treatment along with cyclophosphamide markedly showed improvement in reducing Cyclophosphamide-induced oxidative stress, and normalized sperm characteristics and testicular histopathology. Thus, in conclusion, cyclophosphamide causes fertility changes which leads to male infertility, whereas wheatgrass juice has a protective effect on reversing the testicular damage and male infertility. https://biomedpharmajournal.org/vol16no2/effect-of-wheatgrass-juice-on-fertility-changes-induced-by-cyclophosphamide-in-male-wistar-albino-rats/ Antifertility Antioxidant  Sperm morphology Testicular health