eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2023-06-30 16 2 817 826 10.13005/bpj/2664 49362 article Nisrat Jahan 2 Khozirah Shaari 3 Sk Nazrul Islam 4 ATM Zafrul Azam 1 Fatema-Tuz-Zohora 5 Monira Ahsan 1 Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Dhaka, Dhaka 1000, Bangladesh Department of Pharmacy, southeast University, 251/A and 252, Tejgaon Industrial Area, Dhaka - 1208, Bangladesh Laboratory of Natural Products, Institute of Bioscience, University Putra Malaysia, 43400 Serdang, Selangor, Malaysia 4 Institute of Nutrition and Food Science, University of Dhaka, Dhaka 1000, Bangladesh Department of Pharmacy, University of Asia Pacific, 74/A, Green Road, Dhaka 1205, Bangladesh The study's objectives include phytochemical profiling and biological (antioxidant, thrombolytic and cytotoxic) analysis of pure chemicals from Jatropha pandurifolia stem bark ethyl acetate extract. Five different compounds including octacosanyl cis ferulate (1), hexacosyl (E)-ferulate (2) triacontyl ferulate (3), β-sitosterol (4) and stigmasterol (5) are elucidated. Their structures determine through ¹HNMR analysis and comparison to published data, while three ferulic acid alkyl esters (1-3) were isolated for the first time from J. pandurifolia. Compounds 1, 2, and 3 all have significant thrombolytic potential with respective values of 68.92% ±1.17 (**P<0.01), 66.56% ±2.35 (**P<0.01) and 70.81%±0.98 (**P<0.01) with comparison to standard streptokinase (73.6%±0.76). When compared to BHT (6.82± 0.99 μg/ml) the IC₅₀ (DPPH assay) values were 16.26±1.07 (**P<0.01), 14.12±1.23 (**P<0.01), and 13.16±1.70 μg/ml (**P<0.01). Comparing the three compounds to the reference vincristine sulphate (LC₅₀: 0.52±0.18 μg/ml), of compound 1 (1.56±0.35 μg/ml) (**P<0.01), compound 2 (1.3±0.78 μg/ml) (**P<0.01) and compound 3 (1.29±0.33 μg/ml) (**P<0.01). The results can therefore be interpreted as a concept of isolated molecules having potential for application in additional pharmaceutical research. https://biomedpharmajournal.org/vol16no2/chemical-and-biological-profiling-of-three-ferulic-acids-alkyl-esters-isolated-from-jatropha-pandurifolia-family-euphorbiaceae-stem-bark/ Antioxidant Cytotoxic Activity Hexacosyl (E)-Ferulate Jatropha Pandurifolia Octacosanyl Cis Ferulate Triacontyl Ferulate Thrombolytic