eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2023-03-21 16 1 67 72 10.13005/bpj/2588 47841 article Raden Joko Kuncoroningrat Susilo 1 Dwi Winarni 2 Suhailah Hayaza 1 Sri Puji Astuti Wahyuningsih 2 Ruey-an Doong 3 Win Darmanto 5 Department of Nanotechnology Engineering, Faculty of Advance Technology and Multidiscipline, Universitas Airlangga, Surabaya 60115, Indonesia. Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya 60115, Indonesia. Institute of Analytical and Environmental Sciences, National Tsing Hua University, Sec. 2 Kuang Fu Road, Hsinchu 30013, Taiwan. Department of Pathology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia Institute of Science, Technology, and Health, Jombang 61419, Indonesia Liver fibrosis was regarded as result of wound healing process in chronic liver injury. The α-smooth muscle actin (α-SMA) and matrix metalloproteinase-1 (MMP1) are several indicators for extracellular matrik (ECM) production which relate in fibrosis process. This study aims to determine the effect of Ganoderma applanatum crude polysaccharide (GACP) extract on carbon tetrachloride (CCl₄)-against α-smooth muscle actin and matrix metalloproteinase expressions. A total of 24 male mice strain BALB/C, 3-4 months were divided into 6 groups. control group (distilled water and olive oil), model group (distilled water and CCl₄), low G. applanatum crude polysaccharide group (G. applanatum extract at the dose of 25 mg/kg BW and CCl₄), middle G. applanatum crude polysaccharide group (G. applanatum extract at the dose of 50 mg/kg b.w and CCl₄), high G. applanatum crude polysaccharide group (G. applanatum extract at the dose of 100 mg/kg BW and CCl₄) and silymarin group (silymarin 100 mg/kg BW and CCl₄). Expressions of α-SMA and MMP1 were examined by immunohistochemical methods. The observation of immunohistochemistry used an inverted microscope at 200× magnification. The results of the observation were analyzed using ImageJ software to measure the percentage of α-SMA and MMP1 expressions. All of the data were analyzed by GraphPad Prism Software. The results showed that G. applanatum extracts prevented a significant increase in α-SMA expression and decrease MMP1 expression (p < 0.05) in comparison with the model group. Therefore, G. applanatum extracts play as liver protector against chronic liver injury after successfully inhibit α-SMA expression and prevent reduction of MMP1 expression. https://biomedpharmajournal.org/vol16no1/the-effect-of-ganoderma-applanatum-crude-polysaccharide-against-%ce%b1-smooth-muscle-actin-and-matrix-metalloproteinase-1-expressions-in-mice-after-induced-by-carbon-tetrachloride/ α-Smooth Muscle Actin Fibrosis Ganoderma applanatum Healthy Life Liver Matrix Metalloproteinase