eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2023-03-21 16 1 103 112 10.13005/bpj/2592 47639 article Pavan Udavant 1 Pragati Gurav 1 Gayatri Kanade 1 Neelam Dashputre 1 Rahul sable 1 Shubham Khairnar 1 Department of Pharmacology, MET’s Institute of Pharmacy, Bhujbal Knowledge City, Adgaon, Nashik, Maharashtra, India 422003 Department of Pharmaceutical Chemistry, MET’s Institute of Pharmacy, Bhujbal Knowledge City, Adgaon, Nashik, Maharashtra, India 422003. Introduction: Hepato-renal toxicity is a devastating, non-communicable disease. Because of a lack of information on low-cost management to combat the disease, this study postulates the ameliorative effect of selected phytoconstituents against toxicity. Aim and Objective: The current study reveals an active phytoconstituent, α- Pinene, that has the ability to combat the degenerative effects of CCl4. Methodology: Carbon tetrachloride (CCl₄) is an organic xenobiotic molecule as well as the most potent hepatotoxic agent used (1200 mg/kg body weight; i.p.) to induce hepato-renal toxicity in experimental rats. To determine in vivo hepato-renal toxicity, three different doses (0.05 ml/kg body weight, 0.1 ml/kg body weight, and 0.15 ml/kg body weight; intraperitoneally) were chosen. Vitamin C at the dose of 250 mg/kg/p.o. was used as a standard, due to its maximum ameliorative activity against oxidative damage in CCl₄-induced hepato-renal toxicity in rats. For 7 days, the animals were pre-treated with α-pinene and Vitamin C. CCl₄ was charged only on the 7^th day. Result and Conclusion: The related biochemical tests were studied. CCl₄ intoxication reduces mitochondrial membrane potential in liver and kidney cells, which accelerates excessive intracellular ROS production, but α-pinene pretreatment successfully restores it in both liver and kidney cells. Pretreatment with α-pinene and vitamin C for 7 days increased intracellular ameliorative capability in hepatic and renal cells significantly (p 0.01). In conclusion, α-pinene is capable of restoring antioxidant status by quenching intracellular ROS. As a result, α-pinene has the potential to provide hepatoprotective and nephroprotective effects against CCl4-induced toxicity in rats. https://biomedpharmajournal.org/vol16no1/evaluation-of-hepatoprotective-and-nephroprotective-effect-of-%ce%b1-pinene-on-wistar-albino-rat/ α- Pinene  Biochemical tests CCl4 Hepato-renal toxicity Oxidative damage Vitamin C