eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2022-12-20 15 4 2243 2249 10.13005/bpj/2562 46717 article Nour Athiroh Abdoes Sjakoer 1 Yudi Purnomo 2 Departement of Biology, Faculty of Mathematics and Natural Sciences, Islamic University of Malang, Malang, Indonesia Departement of Pharmacy, Faculty of Medicine, Islamic University of Malang, Malang, Indonesia Hypertension is a degenerative disease that become health problem in the world. S. atropurpurea BI. Dans and D. petandra are herbs that showed anti-hypertension activity. However, the potency of their combination onimmune system in hypertension condition have not been explored completely. The study aim to examine effect of S. atropurpurea BI. Dans and D. petandra combination on Cluster of Differentiation 4 (CD4), Cluster of Differentiation 8(CD8), interleukin-10 (IL-10), and tumor necrosis factor-α (TNF- α) level in hypertension rats. The rat was administrated orally with methanolic extract of S. atropurpurea BI. Dans and D. petandra (1:3) combination at dose of 50 mg/kgBW (P1), 100 mg/kgBW (P2) and 200 mg/kgBW (P3) for four weeks. Hypertension rats are induced with Deoxycorticosterone acetate (DOCA) and salt orally at the second weeks after herbs administration. Blood sample was collected from intra cardiac, furthermore, the level of IL-10 and TNF-α levels are examined using the Enzyme-linked immunosorbent assay (ELISA) method, whereas CD4 and CD8 levels are examined using flow cytometri. The data was analyzed using ANOVA test continued with LSD test (p < 0,05). The treatment groups (PI, P2, and P3) showed an increase of CD4 levels more and less 3 times,10 times, and 8 times respectively; an increase of CD8 level about 2 times, 3 times, and 2 times respectively compare to hypertension group (p<0.05). However, IL-10 level were increased only by P3 group (p<0.05). Meanwhile, the combination of herbsdecreased TNF-α level approximately 50-60% for three doses used (p<0.05). Combination of methanolic extracts of S. atropurpurea BI. Dans and D. petandra increase CD4, CD8 and IL-10 level and decreased TNF-α level on hypertension rat. https://biomedpharmajournal.org/vol15no4/the-effect-of-tea-epiphyte-scurrula-atropurpurea-bi-dans-and-mango-epiphyte-dendrophthoe-petandra-combination-on-cd4-cd8-il-10-and-tnf-%ce%b1-levels-in-hypertension-rats/ Cluster of Differentiation 4 (CD4) Cluster of Differentiation 8(CD8)  D. petandra Hypertension  interleukin-10 (IL-10)  S. atropurpurea BI. Dans  Tumor Necrosis Factor-α (TNF- α)