eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2022-12-20 15 4 2069 2084 10.13005/bpj/2544 46280 article Sharmila Devi Veeraswamy 1 Ilavarasan Raju 2 Sumithra Mohan 1 Department of Pharmacology, SRM College of Pharmacy, SRMIST, Kattankulathur - 603203, Tamil Nadu, India. Captain Srinivasa Murthy Regional Ayurveda Drug Development Institute, Chennai - 600106, Tamil Nadu, India In the current scenario, there is a thirst for research against emerging microorganisms, and it becomes challenging to introduce new drugs against organism virulence are pretty interesting. Herbal medicines are now gaining popularity as a treatment option for various diseases worldwide. The present study analyzes the antifungal effect of a polyherbal formulation through in vitro well diffusion method using fungal strains such as Candida albicans, Aspergillus niger, Aspergillus fumigatus, Cryptococcus neoformans, and Sporothrix schenckii. Molecular docking is done using the Auto dock vina tool to predict the mechanism of action of the phytomolecules present in the polyherbal formulation. The molecular interactions are visualized using molecular modelling (PyMOL) software. The antifungal effect was observed in a concentration-dependent manner with a significant zone of inhibition. Also, phytomolecules in polyherbal formulation showed potential inhibition on CYP450 Lanosterol 14 α-demethylase 1, 3 β-Glucan synthase, and Thymidylate synthase from docking analysis. https://biomedpharmajournal.org/vol15no4/an-approach-to-antifungal-efficacy-through-well-diffusion-analysis-and-molecular-interaction-profile-of-polyherbal-formulation/ Anti-Fungal  Binding energy  Molecular Docking  Polyherbal formulation  well diffusion zone of inhibition