eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2022-06-30 15 2 979 983 10.13005/bpj/2433 44492 article Makkasau Plasay 1 Rosdiana Natzir 3 Muhammad Husni Cangara 4 Marhaen Hardjo 3 Syahrijuita 3 Gita Vita Soraya 3 Master of Biomedical Study Program, Post Graduate School, Hasanuddin University, Makassar, South Sulawesi, Indonesia Panakkukang College School of Health Sciences, Makassar, South Sulawesi, Indonesia Department of Biochemistry, Faculty of Medicine, Hasanuddin University, Makassar, South Sulawesi, Indonesia Department of Anatomical Pathology, Faculty of Medicine, Hasanuddin University, Makassar, South Sulawesi, Indonesia Installation of Anatomical Pathology, Hasanuddin University Hospital, Makassar, South Sulawesi, Indonesia Melittin, one of the cytolytic peptides derived from bee venom, is a broad-spectrum efficacy candidate as an antibacterial, antifungal, and antitumor agent. This study demonstrates the cytotoxic effect of melittin isolated from Apis mellifera through the induction of p53 and 8-OHdG. The antiproliferative effect was evaluated against breast cell cancer MCF-7 via MTT assay, while the molecular mechanism of melittin on MCF-7 was assayed by p53 and 8-OHdG ELISA. With an IC₅₀ value of 5.86 µg/mL ((very toxic)), the cytotoxic impact inhibits MCF-7 cell proliferation in a dose-dependent manner. Significant (p < 0.05) elevations in the level of p53 and 8-OHdG were evident in the IC₅₀-treated cells compared to control. In conclusion, melittin may have considerable potential as a novel natural product-based for breast cancer. https://biomedpharmajournal.org/vol15no2/melittin-induced-cell-death-through-p53-and-8-ohdg-in-breast-cell-cancer-mcf-7/ Cytotoxic  Melittin MCF-7  8-OHdG  p⁵³