eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2022-06-30 15 2 729 746 10.13005/bpj/2411 44049 article Chanchal Bhati 1 Neha Minocha 2 Deepika Purohit 3 Sunil Kumar 3 Manish Makhija 3 Sapna Saini 4 Department of Pharmaceutical Sciences, Gurugram University, Gurugram – 122018, Haryana, India School of Medical and Allied Sciences, K. R. Mangalam University, Gurugram – 122013, Haryana, India Department of Pharmaceutical Sciences, Indira Gandhi University, Meerpur, Rewari – 123401, Haryana, India PDM School of Pharmacy, Karsindhu, Safidon, Jind - 126112, Haryana, India Department of Pharmaceutical Sciences, Maharshi Dayanand University, Rohtak – 124001, Haryana, India Background: Chromatography is defined as a set of techniques that are used for the separation of constituents in a mixture. Introduction: High-Pressure Liquid Chromatography or High-Performance Liquid Chromatography (HPLC) is known as a specialized technique in which columns as well as liquid chromatography are used in the separation, characterizationand investigation of the active moieties existing in the mixture. Objective: Current review focuses on the HPLC technique, including its principles, instrumentation, types, applications, advancements, and patents. Result: HPLC technique is important both for quantitative as well as qualitative analysis and is used for the evaluation of biological and pharmaceutical samples. It is the safest, most versatile, and fastest technique for chromatographic analysis in the field of quality control of drug components. In this review, the authors have also tried to summarize some of the advancements and recent patents in which the HPLC technique was used for the analysis. Conclusion: The article will help in understanding the role and importance of this analytical technique in the quality control of drugs and biologicals. https://biomedpharmajournal.org/vol15no2/high-performance-liquid-chromatography-recent-patents-and-advancement/ Applications Advancements  Chromatography  Detectors  High Performance Liquid Chromatography  Patents  Pumps