eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2022-06-30 15 2 10.13005/bpj/2440 44483 article Ahmad Fauzi 1 Alvarino 2 Yanwirasti 2 Roni Eka Sahputra 2 Student of Doctoral Program in Biomedical Science, Universitas Andalas, Padang, Indonesia Faculty of Medicine, Universitas Andalas, Padang, Indonesia. Faculty of Medicine, Universitas Lampung, Bandar Lampung, Indonesia. One of the causes of nonunion is inadequate vascularization due to severe injury mechanisms that cause defective bone healing factors. Therefore, in non-union fractures, a trigger is needed for the growth factors to work properly. This study aimed to determine the effects of ALSR on  VEGF and CD-34 in non-union fractures. This study used an experimental post-test only control group design that involved white rats of the Sprague-Dawley strain. The VEGF expression was assessed by anti-VEGF staining, which was done by cutting paraffin blocks into a thickness of 5 μm, then the blocks were deparaffinized in xylol and rehydrated with alcohol. The results of the analysis are displayed with their mean ranks between control groups and treatment groups. It was found that CD-34 expression was high in the ALSR group. This shows the occurrence of angiogenesis and the regeneration in the case of non-union fractures in this study. https://biomedpharmajournal.org/vol15no2/effects-of-amnion-lyophilization-sterile-radiation-on-vascular-endothelial-growth-factor-and-cluster-of-differentiation-34-in-nonunion-fractures/ Amnion Lyophilization Sterile Radiation  Cluster of Differentiation 34  Nonunion fractures  Vascular Endothelial Growth Factor