eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2022-03-31 15 1 73 81 10.13005/bpj/2344 43021 article Rosamarlina 1 Mochammad Hatta 2 Irawaty Djaharuddin 4 Ilhamjaya Patellongi 5 Agus Dwi Susanto 6 Andi Asadul Islam 7 Sulianti Saroso Infectious Diseases Hospital, Jakarta, Indonesia Faculty of Medicine, Hasanuddin University, Makassar, Indonesia Molecular Biology and Immunology Laboratory, Faculty of Medicine Hasanuddin University, Makassar, Indonesia. Department of Pulmonology and Respiratory Medicine, Faculty of Medicine Hasanuddin University, Makassar, Indonesia. Department of Physiology, Faculty of Medicine Hasanuddin University, Makassar, Indonesia. Department of Pulmonology and Respiratory Medicine, Persahabatan Hospital, Jakarta, Indonesia. Background: Increasing resistance to TB drugs raises the challenge of TB eradication. Miana leaves is Indonesian traditional herbal medicine, have antimicrobial, anti-inflammatory, and immunoregulatory action. Not much is known about the effect of Miana on HIF-1α and ICAM-1, the immunoregulators of infection and inflammation. This study aims to elucidate the effect of Miana on HIF-1α and ICAM-1 in M. tuberculosis (Mtb) infected mice. Materials and Methods: This experimental study used Mtb infected Balb/c mice were divided into 4 groups; group 1 is placebo, group 2 is treated with Rifampicin as Anti TB drug, group 3 is treated with Miana, and group 4 is treated with Miana + Anti TB drug. HIF-1α and ICAM-1 serum levels were analyzed using ELISA. Results: There is a significant difference of mean HIF-1α (p= 0.00, F = 114.21) and ICAM-1 (p= 0.00, F = 113.11) between the four groups after treatment. HIF-1α level is significantly lower in anti TB treatment, Miana, and Miana + anti TB treatment compared to placebo (mean difference (MD) 35,764.67, p=0.00; 29,230.98, p=0.000; 38,489.62, p=0.00, respectively). Furthermore, ICAM-1 level is significantly lower in anti TB treatment, Miana, and Miana + anti TB treatment compared to placebo (MD 95,449.68, p=0.00; 79,509.69, p=0.00; 108,672.83, p=0.00, respectively). Conclusion: HIF-1α and ICAM-1 expression was reduced after Miana administration. Miana can be a potential complement to anti-TB treatment but cannot replace rifampicin as anti-TB drugs. https://biomedpharmajournal.org/vol15no1/the-changes-of-hif-1%ce%b1-and-icam-1-expression-after-miana-coleus-scutellariodes-l-treatment-in-balbc-mice-with-mycobacterium-tuberculosis-infection/ Coleus scutellariodes  HIF-1α ICAM-1  Miana leaf  M. tuberculosis  Rifampicin