eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2022-03-31 15 1 469 476 10.13005/bpj/2387 42879 article Prasad Konduri 1 Eswar Kumar Kilari 2 Ravindra Babu Sajja 3 Department of Pharmacology, Shri Vishnu College of Pharmacy, Bhimavaram. Department of Pharmacology , College of Pharmaceutical Sciences, Andhra University, Visakhapatnam. Department of Pharmacology, Malla Reddy Institute of Pharmaceutical Sciences, Secunderabad. Objective: The objective of the present work is to evaluate the petroleum ether, ethyl acetate and methanolic extracts of vanda spathulata on experimental models for in vitro antioxidant activity and mast cell stabilizing activity. Methods: Mast cell stabilization effect was assessed using compound 48/80 induced mast cell degranulation in rat peritoneal mast cell. The antioxidant activity was evaluated using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay. Results and Discussion: Vanda spathulata methanolic extract (VSME) at doses of 200 and 400mg/kg exhibited significant protection (p<0.01) and petroleum ether (VSPE) and ethylacetate (VSEA) extracts at the dose of 400mg/kg showed significant protection (p<0.05) against mast cell degranulation. VSME revealed better DPPH radical scavenging activity (IC₅₀ value 38.39 µg/ml) which was closely resembled to standard ascorbic acid (IC₅₀ value 33.98 µg/ml) when compared to other extracts. Phytochemical study revealed the presence of alkaloids, saponins, flavonoids, tannins, steroids and glycosides. Conclusion: From the results Vanda spathulata shows mast cell stabilizing activity mainly due to phytochemical constituents and strong antioxidant property of plant extracts. https://biomedpharmajournal.org/vol15no1/in-vitro-antioxidant-and-mast-cell-stabilizing-activity-of-different-extracts-of-vanda-spathulata-flowers/ Antioxidant  Comp 48/80  DPPH  Flavonoids  Mast cell degranulation  Vanda spathulata