eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2022-03-31 15 1 33 47 10.13005/bpj/2341 42950 article Jihan Hussein 1 Mona El Bana 1 Dalia Medhat 1 Yasmin Abdel Latif 1 Samah M. El- sayed 2 Ahmed M. Youssef 3 Medical Biochemistry Department, Medical Research Division, National Research Centre, Dokki, Giza, P.O. 12622 Egypt Dairy Science Department, National Research Centre, Dokki, Giza, P.O. 12622 Egypt Packing and Packaging Materials Department, National Research Centre, 12622, Dokki, Egypt Textile Research Division, National Research Centre, Dokki, Giza, P.O. 12622 Egypt Hibiscus sabdariffa L. has been widely cultivated in tropical areas, usually used in treatment of many disorders. Thus, in our study we aimed to evaluate the effect of dairy desserts supplemented with nanoform of Hibiscus sabdariffa L. extract (NHSE) against Ehrlich solid carcinoma (ESC) in mice. The NHSE was prepared by soaking the fine powder of plant in 90% ethanol by cold extraction. NHSE was evaluated using dynamic light scattering (DLS) and transmission electron microscope (TEM), then the prepared NHSE was added to dairy desserts using different concentrations.  Sixty female albino mice were used and divided into six groups. After the end of the experimental period, blood was withdrawn; Serum was separated for determination of malondialdehyde (MDA), super oxidedismutase (SOD), catalase (CAT), tumor necrosis factor- α (TNF-α), matrix metalloproteinases-9 (MMP-9) and B-cell lymphoma-2 (Bcl-2). Serum homocystein (Hcy) level was estimated by high performance liquid chromatography (HPLC). Mice inoculated intramuscularly with Ehrlich cell line showed statistically marked increase in serum levels of MDA, TNF-α, MMP-9 and Hcy accompanied by marked decrease in SOD and CAT activities and Bcl-2 levels compared to the control group. Treatments with NHSE markedly triggered activity of anti-oxidant, attenuated the inflammatory response, reduced levels of Hcy and stimulated the apoptosis of tumor cells. Based on that, dairy desserts containing NHSE showed effective role in prohibiting the releasing of reactive oxygen species, ameliorating the immune response, and preventing tumor progression. https://biomedpharmajournal.org/vol15no1/hibiscus-sabdariffa-l-nanoparticles-offer-a-preventive-potential-against-experimental-ehrlich-solid-carcinoma/ Dairy Desserts  Ehrlich solid carcinoma Hibiscus sabdariffa L nano-extract Homocysteine  Matrix Metalloproteinases-9