eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2022-03-31 15 1 313 320 10.13005/bpj/2369 42675 article Mutiara Rahmah Amari 1 Hesti Lina Wiraswati 1,2 Nisa Fauziah 2,3 Ilma Fauziah Ma’ruf 4 Oncology and Stem Cells Working Group, Faculty of Medicine, Universitas Padjadjaran, Bandung Infections Working Group, Faculty of Medicine, Universitas Padjadjaran, Bandung Parasitology Division, Department of Biomedical Sciences, Faculty of Medicine, Universitas Padjadjaran, Bandung Biochemistry Research Group, Department of Chemistry, Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung, Bandung Plasmodium falciparum is the most common species of Plasmodium that causes malaria in Southeast Asia. Artemisinin, a drug with the mechanism of action by inducing oxidative stress in infected red blood cells (RBC) is currently used as the main therapy for malaria, after resistance to chloroquine has been found. However, evidence of artemisinin resistance was discovered in several regions in Southeast Asia. Therefore, a research is required to prove the existence of other drugs that have anti-malaria effects. A drug candidate, doxorubicin also can induce the formation of oxidative stress inside the cells. This study aims to determine the activity of doxorubicin to inhibit the development of P. falciparum in vitro. Red blood cell (RBC) infected with P. falciparum were treated with various concentrations of doxorubicin. Giemsa technique was applied to detect P. falciparum inside RBC. After 48 hours of incubation, the culture was observed to measure the number and the confluence of RBC and P. falciparum in the medium. This study revealed that doxorubicin reduced the number of RBC infected with P. falciparum lysis. The effective dose of doxorubicin-inhibit RBC cell lysis is 0.4 μM, which only reduces 81% RBC cell lysis compared to the control group that reduces 95% RBC cell lysis. At this concentration also found a decrease in the number of P. falciparum cells in the medium. The results proved that doxorubicin has an inhibitory effect on the development of P. falciparum and can decrease the lysis of RBC due to P. falciparum infection. This findings provide an insight that doxorubicin is a potential candidate for antimalarial drugs. https://biomedpharmajournal.org/vol15no1/antimalarial-effect-of-doxorubicin-on-plasmodium-falciparum-an-in-vitro-study-in-fcr-3-strain/ Artemisinin Antimalaria   Oxidative Stress Giemsa