eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2021-12-30 14 4 2047 2062 10.13005/bpj/2302 42303 article Suliman A. Alderhami 1 Khaled A. Abdelshafeek 1 Walid E. Abdallah 2 Ahmed A. Elhenawy 1 AbdulAziz Ali Alomari 1 Department of chemistry, faculty of sciences and arts, Mukhwah , Albaha university, Albaha, Saudi Arabia 2 Department chemistry of medicinal plants, Pharmaceutical Industries Div., National Research Centre,33 El Buhouth St.(Former El Tahrir St.), 12622-Dokki, Giza, Egypt. Chemistry Department, Faculty of Science, Al-Azhar University (Boys Branch),Nasr City, Cairo, Egypt. Glucosinolates (Gls) are natural bioactive compounds that form metabolites called isothiocyanates (ITC) which have various therapeutic effects. This study aimed to isolate the glucosinolates of Carrichtera annua L.(DC) (CA) and Farsetia aegyptia Turra (FA) belonging to the Crucifereae family. Total Gls were isolated from the aqueous methanolic extract of the plants and further purified using an acidic aluminum oxide column. Some of the obtained Gls were identified via spectroscopic methods (UV, NMR, and MS) and the rest were hydrolyzed by myrosinase to the corresponding isothiocyanates (ITC) for identification by GC/MS. Only one Gls was identified in CA as 4-methylthio-3-butenyl Gls (MTBG) in addition to 6-methyl sulfonylhexyl isothiocyanates (ITC), while 6-methyl sulfonyl-6-hydroxy hexyl ITC, 4-pentenyl ITC, 3-methylthio propyl ITC, 5-hydroxy pentyl ITC and 4-methylsulphinyl butyl ITC were identified in FA. The Gls demonstrated high binding activity to α-glucosidase and amylase, good pharmacokinetic characteristics, and exerted no carcinogenetic effects. https://biomedpharmajournal.org/vol14no4/glucosinolates-with-their-hydrolysis-products-from-two-cruciferous-plants-with-study-of-antidiabetic-activity-based-on-molecular-docking/ Crucifereae Carrichtera annua  Docking Farsetia aegyptia  Glucosinolates and Isothiocyanates  GC/MS