eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2021-09-30 14 3 1405 1413 10.13005/bpj/2243 40886 article P. Selvi 1 S. Murugesh 1 R. Yuvarajan 2 A. Rajasekar 2 Department of Botany, School of Life Sciences, Periyar University, Salem, Tamil Nadu, India Department of Biotechnology, Mahendra Arts and Science College, Kalippatti, Namakkal, Tamil Nadu, India. Department of Biotechnology, SRM Institute of Science and Technology, Ramapuram Campus, Chennai, India. Modern lifestyle, pollution, food habit, and stress have intensively enhanced the evolution of several diseases on human being. Medicinal plants are used from the ancient times for the therapeutic needs to cure various diseases as well as less toxic in nature. One of such plant is Cissus arnottiana which is used from the olden days which has been identified as an important medicine plant by many researchers all over the world. Cissus arnottiana contains phytochemicals such as alkaloids, tannins, terpenoids, flavonoids, phenolic compounds etc. In this present work methanolic stem extract of Cissus arnottiana is used to evaluate the antibacterial activity of gram negative and gram positive human pathogenic bacteria. DPPH assay is used to investigate the antioxidant properties of the methanolic extract. The anti-inflammatory activity of the extract has been studied using anti proteinase assay. The MTT cell proliferation assay is carried out against HeLa cell line in which 44% of cells are viable for the concentration of 100 µg/mL. Interestingly, the methanolic stem extract can be used as a potential candidate for new therapeutic applications. https://biomedpharmajournal.org/vol14no3/screening-the-therapeutic-potential-of-methanolic-stem-extract-of-cissus-arnottiana/ Cissus arnottiana  Cell Lines  Plants Extract  Phytochemicals