eng Oriental Scientific Publishing Company Biomedical and Pharmacology Journal 0974-6242 2021-09-30 14 3 1707 1720 10.13005/bpj/2272 41024 article Ruby George 1 Priti Mathur 1 Chandni Tandon 2 Amity Institute of Biotechnology, Amity University Uttar Pradesh, Lucknow, India – 226 028 Patanjali Research Institute, Haridwar, Uttarakhand, India 249405 Background: Dinoxin B Withanolide was isolated from Datura inoxia and identified with its cytotoxic activity. But its antibacterial properties are not yet evaluated. We have previously reported the broad-spectrum antibacterial property of Dinoxin B Withanolide extracted from D.inoxia on standard strains. Objective: This research has focused to evaluate the efficacy of Dinoxin B Withanolide against infectious Staphylococcus aureus, including resistant strains. Methods: Electrospray Ionization-Mass Spectrometry is used to depict the presence of Dinoxin B withanolide from the chromatographic ethanolic leaf fraction. Antibacterial activity of different concentrations of  Dinoxin B(12500-100000 μg/ml) was assessed using the agar diffusion, macro broth dilution, and time-kill assay methods. Docking studies and Drug likeness properties were analyzed. Result: Electrospray Ionization-Mass Spectrometry depicted the presence of Dinoxin B. All the isolates were susceptible to Dinoxin B within the range of 15±0.5mm to 24±0.5mm, and the bacteria were susceptible at a concentration rate of ≤12.5mg/ml. Time-kill assay showed that 25mg/ml of Dinoxin B displayed the highest inhibitory activity after four hours. The MBC values were compatible with the cidal concentration as seen in the time-kill study's growth curve. Computer-aided techniques resulted in a good  Docking score towards Quorum-signaling Sar A protein (-7.82)and Penicillin Binding Protein(-6.9). Conclusion: Dinoxin B with its bactericidal properties and significant affinity towards  Quorum-signaling Sar A protein and Penicillin Binding Protein can be considered as an effective bioactive compound against Methicillin Resistance Staphylococcus aureus. https://biomedpharmajournal.org/vol14no3/dinoxin-b-withanolide-from-datura-inoxia-mill-as-an-effective-phytocompound-against-urinary-tract-infection-causing-staphylococcus-aureus/ Antibacterial activity  Datura inoxia  Dinoxin B Withanolide  Molecular docking Methicillin-Resistant  Staphylococcus aureus