Background and objectives: The important component of all antibiotic stewardship program is education of clinicians about the antimicrobial usage. The objective of the study was to evaluate the knowledge and attitudes regarding antibiotic resistance among medical doctors in a tertiary care hospital. The other objective was to evaluate the usefulness of the interactive educational intervention in improving the knowledge about antibiotic resistance

Methodology: This was a cross sectional questionnaire based study involving residents and postgraduates from various departments. Questionnaire had three categories of assessment, namely, knowledge about the development of antimicrobial resistance (fifteen questions), treatment of resistant microbial conditions (five questions) and prevention of antimicrobial resistance (five questions). Questionnaire was pre-tested and pilot run.

After the filling up of the questionnaire, each participant or a group of three or four participants were provided with interactive educational intervention. In each such session, three components were discussed using a custom designed course module using a learning management system. The modes of development of antimicrobial resistance, methods of rationale antimicrobial prescription and interventions to prevent development of antimicrobial resistance were the main components in the online module. After the online course, each participant filled post-test. The long-term retention of the concepts was tested with one-month follow-up post-test.

Results: 120 participants have taken up the pre-test and undergone the study. Pre-test average score was 11.1(± 3.44) out of 25. Of these participants 114, have taken immediate post-test and average score was 20.9 (± 2.67). The improvement of pre-test scores to post-test scores was statistically significant. 94 participants have answered the one-month post-test, the average score was 16 (± 4.79). The results were statistically significantly more than the pre-test score, however statistically lower than post-test score.

Conclusion: Use of the interactive online learning material has resulted in significant improvement in the knowledge and attitude regarding antibiotic resistance among post-graduates and interns in a tertiary care hospital.