Bio-impedance diagnosis depends mainly on two factors first being the location of electrode placement and second being the distance between electrodes. To minimize the errors caused during bio impedance measurement at variable frequencies, it is required to study the electrodes used for the measurement and its exact location where the electrodes are placed. This paper analyses various electrode separations used for bioimpedance measurement for cardiac signal. The electrode system is used based on the type of tissue under consideration for measurement. It is found that the errors are dependent on the frequencies at which the measurement is carried out and it also depends on the intensity of current applied. Thus, selection of current and frequency is very important for measurements. In this paper, the impact of size, distance and location of the electrodes that can be used for bio-impedance cardiograph.