Introduction: Overweight and obesity prevalence continues to increase and worldwide growing epidemic health problems. Obesity imposes a significant effect on liver functions that may be associated with a substantial risk factor for the development of several non-communicable diseases, considerable disability, and premature death.

Objective: To estimate and compare serum activity of ALT, ALP, and GGT among healthy and obese Sudanese individuals. Materials and methods: a total number of 80 participants were enrolled in this cross-sectional study, and they were categorized into 40 obese and 40 normal-weight individuals. Serum liver enzyme activity was measured for each participant.Results: A significant increase was found in ALT, ALP, and GGT levels among the obese group. There is a statistical difference of GGT level mean between gender within the obese group. A positive correlation was observed between the BMI and serum level of ALT (r=0.343, p =0.002), ALP (r=0.503, p <0.001) and GGT (r=0.237, p= 0.034) and positive correlation between obesity duration and GGT level (r=0.461, p =0.003).Conclusion: Study results revealed that obesity was associated with a substantial elevation in liver enzymes that considered risk factors for liver diseases in Sudanese individuals.